4x lithium dodecyl sulfate lds loading buffer (Thermo Fisher)

Thermo Fisher is a verified supplier

Thermo Fisher manufactures this product

About

News

Press Release

Team

Advisors

Partners

Contact

Bioz Stars

Bioz vStars

Buy from Supplier

Structured Review

Thermo Fisher 4x lithium dodecyl sulfate lds loading buffer
4x Lithium Dodecyl Sulfate Lds Loading Buffer, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/lithium+loading+buffer/LDS-sample+buffer+(4X)%2C+reducing/pmc13138722-180-12-19
Average 99 stars, based on 1 article reviews

4x lithium dodecyl sulfate lds loading buffer - by Bioz Stars, 2026-11

99/100 stars

Images

other:

Article Title: Mapping variants in thyroid hormone transporter MCT8 to disease severity by genomic, phenotypic, functional, structural and deep learning integration
Article Snippet: Cell surface proteins were isolated using Neutravidin agarose beads (Thermo Fisher Scientific) and eluted in NuPAGE 1x lithium dodecyl sulfate (LDS) loading buffer (Thermo Fisher Scientific) containing 10 mM DTT by incubating the beads for 5 min at 90 °C prior to immunoblot analyses.

SDS-Gel:

Article Title: Two cooperative lipid binding sites within the pleckstrin homology domain are necessary for AKT binding and stabilization to the plasma membrane.
Article Snippet: .. Thirty-five micrograms of protein lysate were combined with NUPAGE lithium dodecyl sulfate (LDS sample loading buffer (Thermo Fisher, UK, CatNP0007) and dithiothreitol (DTT) reducing agent (Thermo Fisher, UK, Cat#NP0004) and were heated for 10 min at 70 C. The denatured protein lysates were loaded onto a 12% SDS gel and electrophoresed at 80 V for one hour and then at a constant of 120 V and run until the loading dye front reached the bottom of the gel. .. Following electrophoresis, the protein samples were transferred onto a methanol-activated polyvinylidene fluoride (PVDF) membrane (Merck, UK, Cat#IPFL00010).

Virus:

Article Title: Two cooperative lipid binding sites within the pleckstrin homology domain are necessary for AKT binding and stabilization to the plasma membrane.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies AKT (pan) (40D4) mouse mAb Cell Signalling Technology Cat#2920; RRID:AB_1147620 Phospho-Akt (Ser473) (193H12) Rabbit mAb Cell Signalling Technology Cat#4058; RRID:AB_331168 b-Actin (8H10D10) Mouse mAb Cell Signalling Technology Cat#3700; RRID:AB_2242334 IRDye 800CV goat anti-mouse Cell Signalling Technology Cat#926-68170; RRID:AB_10956589 IRDye 680RD goat anti-rabbit LI-COR Biosciences Cat#926-68071; RRID:AB_10956166 Bacterial and virus strains E.coli DH10B competent cells Invitrogen Cat#1296606; N/A Chemicals, peptides, and recombinant proteins ampicillin Sigma–Aldrich Cat#A9518; N/A Lipofectamine 3000 Thermo Fisher Cat#L3000015; N/A ProLong Diamond Antifade Mountant with DAPI Thermo Fisher Cat#17625062; N/A HALT Protease & Phosphatase Inhibitor Cocktail (100x) Thermo Fisher Cat#861281; N/A NUPAGE lithium dodecyl sulfate (LDS sample loading buffer Thermo Fisher Cat#NP0007; N/A dithiothreitol (DTT) reducing agent Thermo Fisher Cat#NP0004; N/A methanol-activated polyvinylidene fluoride (PVDF) membrane Merck Cat#IPFL00010; N/A tris-buffered saline (TBS) Odyssey Blocking Buffer LI-COR Biosciences Cat#92750000; N/A Perifosine Sigma-Aldrich Cat#SML0612; N/A Critical commercial assays HiSpeed Plasmid Maxi Kit Qiagen Cat#12663; N/A Pierce BCA Protein Assay Kit Thermo Fisher Cat#23227; N/A Deposited data Molecular dynamics simulation trajectories This paper https://doi.org/10.5518/1313 High resolution structure of AKT-PH Milburn et al. PDB: 1UNQ Experimental models: Cell lines .. Recombinant DNA Plasmid: AKTWT-mCherry (See Table S1) This paper N/A Plasmid: AKTWT-EGFP (See Table S1) This paper N/A Plasmid: AKTE17K-EGFP (See Table S1) This paper N/A Plasmid: AKTmut1-EGFP (See Table S1) This paper N/A Plasmid: AKTmut2-EGFP (See Table S1) This paper N/A Plasmid: AKTmut3-EGFP (See Table S1) This paper N/A Plasmid: AKT-PHmut3-mCherry (See Table S1) This paper N/A pcDNA3.1 empty vector (See Table S1) Gifted by Dr. Thomas Hughes, University of Leeds Addgene plasmid # 40728; RRID: Addgene_40728 HA-GFP reporter (See Table S1) Gifted by Dr. Thomas Hughes, University of Leeds N/A (Continued on next page) e1 Structure 33, 1–15.e1–e5, January 2, 2025

Recombinant:

Article Title: Two cooperative lipid binding sites within the pleckstrin homology domain are necessary for AKT binding and stabilization to the plasma membrane.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies AKT (pan) (40D4) mouse mAb Cell Signalling Technology Cat#2920; RRID:AB_1147620 Phospho-Akt (Ser473) (193H12) Rabbit mAb Cell Signalling Technology Cat#4058; RRID:AB_331168 b-Actin (8H10D10) Mouse mAb Cell Signalling Technology Cat#3700; RRID:AB_2242334 IRDye 800CV goat anti-mouse Cell Signalling Technology Cat#926-68170; RRID:AB_10956589 IRDye 680RD goat anti-rabbit LI-COR Biosciences Cat#926-68071; RRID:AB_10956166 Bacterial and virus strains E.coli DH10B competent cells Invitrogen Cat#1296606; N/A Chemicals, peptides, and recombinant proteins ampicillin Sigma–Aldrich Cat#A9518; N/A Lipofectamine 3000 Thermo Fisher Cat#L3000015; N/A ProLong Diamond Antifade Mountant with DAPI Thermo Fisher Cat#17625062; N/A HALT Protease & Phosphatase Inhibitor Cocktail (100x) Thermo Fisher Cat#861281; N/A NUPAGE lithium dodecyl sulfate (LDS sample loading buffer Thermo Fisher Cat#NP0007; N/A dithiothreitol (DTT) reducing agent Thermo Fisher Cat#NP0004; N/A methanol-activated polyvinylidene fluoride (PVDF) membrane Merck Cat#IPFL00010; N/A tris-buffered saline (TBS) Odyssey Blocking Buffer LI-COR Biosciences Cat#92750000; N/A Perifosine Sigma-Aldrich Cat#SML0612; N/A Critical commercial assays HiSpeed Plasmid Maxi Kit Qiagen Cat#12663; N/A Pierce BCA Protein Assay Kit Thermo Fisher Cat#23227; N/A Deposited data Molecular dynamics simulation trajectories This paper https://doi.org/10.5518/1313 High resolution structure of AKT-PH Milburn et al. PDB: 1UNQ Experimental models: Cell lines .. Recombinant DNA Plasmid: AKTWT-mCherry (See Table S1) This paper N/A Plasmid: AKTWT-EGFP (See Table S1) This paper N/A Plasmid: AKTE17K-EGFP (See Table S1) This paper N/A Plasmid: AKTmut1-EGFP (See Table S1) This paper N/A Plasmid: AKTmut2-EGFP (See Table S1) This paper N/A Plasmid: AKTmut3-EGFP (See Table S1) This paper N/A Plasmid: AKT-PHmut3-mCherry (See Table S1) This paper N/A pcDNA3.1 empty vector (See Table S1) Gifted by Dr. Thomas Hughes, University of Leeds Addgene plasmid # 40728; RRID: Addgene_40728 HA-GFP reporter (See Table S1) Gifted by Dr. Thomas Hughes, University of Leeds N/A (Continued on next page) e1 Structure 33, 1–15.e1–e5, January 2, 2025

Membrane:

Article Title: Two cooperative lipid binding sites within the pleckstrin homology domain are necessary for AKT binding and stabilization to the plasma membrane.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies AKT (pan) (40D4) mouse mAb Cell Signalling Technology Cat#2920; RRID:AB_1147620 Phospho-Akt (Ser473) (193H12) Rabbit mAb Cell Signalling Technology Cat#4058; RRID:AB_331168 b-Actin (8H10D10) Mouse mAb Cell Signalling Technology Cat#3700; RRID:AB_2242334 IRDye 800CV goat anti-mouse Cell Signalling Technology Cat#926-68170; RRID:AB_10956589 IRDye 680RD goat anti-rabbit LI-COR Biosciences Cat#926-68071; RRID:AB_10956166 Bacterial and virus strains E.coli DH10B competent cells Invitrogen Cat#1296606; N/A Chemicals, peptides, and recombinant proteins ampicillin Sigma–Aldrich Cat#A9518; N/A Lipofectamine 3000 Thermo Fisher Cat#L3000015; N/A ProLong Diamond Antifade Mountant with DAPI Thermo Fisher Cat#17625062; N/A HALT Protease & Phosphatase Inhibitor Cocktail (100x) Thermo Fisher Cat#861281; N/A NUPAGE lithium dodecyl sulfate (LDS sample loading buffer Thermo Fisher Cat#NP0007; N/A dithiothreitol (DTT) reducing agent Thermo Fisher Cat#NP0004; N/A methanol-activated polyvinylidene fluoride (PVDF) membrane Merck Cat#IPFL00010; N/A tris-buffered saline (TBS) Odyssey Blocking Buffer LI-COR Biosciences Cat#92750000; N/A Perifosine Sigma-Aldrich Cat#SML0612; N/A Critical commercial assays HiSpeed Plasmid Maxi Kit Qiagen Cat#12663; N/A Pierce BCA Protein Assay Kit Thermo Fisher Cat#23227; N/A Deposited data Molecular dynamics simulation trajectories This paper https://doi.org/10.5518/1313 High resolution structure of AKT-PH Milburn et al. PDB: 1UNQ Experimental models: Cell lines .. Recombinant DNA Plasmid: AKTWT-mCherry (See Table S1) This paper N/A Plasmid: AKTWT-EGFP (See Table S1) This paper N/A Plasmid: AKTE17K-EGFP (See Table S1) This paper N/A Plasmid: AKTmut1-EGFP (See Table S1) This paper N/A Plasmid: AKTmut2-EGFP (See Table S1) This paper N/A Plasmid: AKTmut3-EGFP (See Table S1) This paper N/A Plasmid: AKT-PHmut3-mCherry (See Table S1) This paper N/A pcDNA3.1 empty vector (See Table S1) Gifted by Dr. Thomas Hughes, University of Leeds Addgene plasmid # 40728; RRID: Addgene_40728 HA-GFP reporter (See Table S1) Gifted by Dr. Thomas Hughes, University of Leeds N/A (Continued on next page) e1 Structure 33, 1–15.e1–e5, January 2, 2025

Saline:

Article Title: Two cooperative lipid binding sites within the pleckstrin homology domain are necessary for AKT binding and stabilization to the plasma membrane.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies AKT (pan) (40D4) mouse mAb Cell Signalling Technology Cat#2920; RRID:AB_1147620 Phospho-Akt (Ser473) (193H12) Rabbit mAb Cell Signalling Technology Cat#4058; RRID:AB_331168 b-Actin (8H10D10) Mouse mAb Cell Signalling Technology Cat#3700; RRID:AB_2242334 IRDye 800CV goat anti-mouse Cell Signalling Technology Cat#926-68170; RRID:AB_10956589 IRDye 680RD goat anti-rabbit LI-COR Biosciences Cat#926-68071; RRID:AB_10956166 Bacterial and virus strains E.coli DH10B competent cells Invitrogen Cat#1296606; N/A Chemicals, peptides, and recombinant proteins ampicillin Sigma–Aldrich Cat#A9518; N/A Lipofectamine 3000 Thermo Fisher Cat#L3000015; N/A ProLong Diamond Antifade Mountant with DAPI Thermo Fisher Cat#17625062; N/A HALT Protease & Phosphatase Inhibitor Cocktail (100x) Thermo Fisher Cat#861281; N/A NUPAGE lithium dodecyl sulfate (LDS sample loading buffer Thermo Fisher Cat#NP0007; N/A dithiothreitol (DTT) reducing agent Thermo Fisher Cat#NP0004; N/A methanol-activated polyvinylidene fluoride (PVDF) membrane Merck Cat#IPFL00010; N/A tris-buffered saline (TBS) Odyssey Blocking Buffer LI-COR Biosciences Cat#92750000; N/A Perifosine Sigma-Aldrich Cat#SML0612; N/A Critical commercial assays HiSpeed Plasmid Maxi Kit Qiagen Cat#12663; N/A Pierce BCA Protein Assay Kit Thermo Fisher Cat#23227; N/A Deposited data Molecular dynamics simulation trajectories This paper https://doi.org/10.5518/1313 High resolution structure of AKT-PH Milburn et al. PDB: 1UNQ Experimental models: Cell lines .. Recombinant DNA Plasmid: AKTWT-mCherry (See Table S1) This paper N/A Plasmid: AKTWT-EGFP (See Table S1) This paper N/A Plasmid: AKTE17K-EGFP (See Table S1) This paper N/A Plasmid: AKTmut1-EGFP (See Table S1) This paper N/A Plasmid: AKTmut2-EGFP (See Table S1) This paper N/A Plasmid: AKTmut3-EGFP (See Table S1) This paper N/A Plasmid: AKT-PHmut3-mCherry (See Table S1) This paper N/A pcDNA3.1 empty vector (See Table S1) Gifted by Dr. Thomas Hughes, University of Leeds Addgene plasmid # 40728; RRID: Addgene_40728 HA-GFP reporter (See Table S1) Gifted by Dr. Thomas Hughes, University of Leeds N/A (Continued on next page) e1 Structure 33, 1–15.e1–e5, January 2, 2025

Blocking Assay:

Article Title: Two cooperative lipid binding sites within the pleckstrin homology domain are necessary for AKT binding and stabilization to the plasma membrane.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies AKT (pan) (40D4) mouse mAb Cell Signalling Technology Cat#2920; RRID:AB_1147620 Phospho-Akt (Ser473) (193H12) Rabbit mAb Cell Signalling Technology Cat#4058; RRID:AB_331168 b-Actin (8H10D10) Mouse mAb Cell Signalling Technology Cat#3700; RRID:AB_2242334 IRDye 800CV goat anti-mouse Cell Signalling Technology Cat#926-68170; RRID:AB_10956589 IRDye 680RD goat anti-rabbit LI-COR Biosciences Cat#926-68071; RRID:AB_10956166 Bacterial and virus strains E.coli DH10B competent cells Invitrogen Cat#1296606; N/A Chemicals, peptides, and recombinant proteins ampicillin Sigma–Aldrich Cat#A9518; N/A Lipofectamine 3000 Thermo Fisher Cat#L3000015; N/A ProLong Diamond Antifade Mountant with DAPI Thermo Fisher Cat#17625062; N/A HALT Protease & Phosphatase Inhibitor Cocktail (100x) Thermo Fisher Cat#861281; N/A NUPAGE lithium dodecyl sulfate (LDS sample loading buffer Thermo Fisher Cat#NP0007; N/A dithiothreitol (DTT) reducing agent Thermo Fisher Cat#NP0004; N/A methanol-activated polyvinylidene fluoride (PVDF) membrane Merck Cat#IPFL00010; N/A tris-buffered saline (TBS) Odyssey Blocking Buffer LI-COR Biosciences Cat#92750000; N/A Perifosine Sigma-Aldrich Cat#SML0612; N/A Critical commercial assays HiSpeed Plasmid Maxi Kit Qiagen Cat#12663; N/A Pierce BCA Protein Assay Kit Thermo Fisher Cat#23227; N/A Deposited data Molecular dynamics simulation trajectories This paper https://doi.org/10.5518/1313 High resolution structure of AKT-PH Milburn et al. PDB: 1UNQ Experimental models: Cell lines .. Recombinant DNA Plasmid: AKTWT-mCherry (See Table S1) This paper N/A Plasmid: AKTWT-EGFP (See Table S1) This paper N/A Plasmid: AKTE17K-EGFP (See Table S1) This paper N/A Plasmid: AKTmut1-EGFP (See Table S1) This paper N/A Plasmid: AKTmut2-EGFP (See Table S1) This paper N/A Plasmid: AKTmut3-EGFP (See Table S1) This paper N/A Plasmid: AKT-PHmut3-mCherry (See Table S1) This paper N/A pcDNA3.1 empty vector (See Table S1) Gifted by Dr. Thomas Hughes, University of Leeds Addgene plasmid # 40728; RRID: Addgene_40728 HA-GFP reporter (See Table S1) Gifted by Dr. Thomas Hughes, University of Leeds N/A (Continued on next page) e1 Structure 33, 1–15.e1–e5, January 2, 2025

Plasmid Preparation:

Article Title: Two cooperative lipid binding sites within the pleckstrin homology domain are necessary for AKT binding and stabilization to the plasma membrane.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies AKT (pan) (40D4) mouse mAb Cell Signalling Technology Cat#2920; RRID:AB_1147620 Phospho-Akt (Ser473) (193H12) Rabbit mAb Cell Signalling Technology Cat#4058; RRID:AB_331168 b-Actin (8H10D10) Mouse mAb Cell Signalling Technology Cat#3700; RRID:AB_2242334 IRDye 800CV goat anti-mouse Cell Signalling Technology Cat#926-68170; RRID:AB_10956589 IRDye 680RD goat anti-rabbit LI-COR Biosciences Cat#926-68071; RRID:AB_10956166 Bacterial and virus strains E.coli DH10B competent cells Invitrogen Cat#1296606; N/A Chemicals, peptides, and recombinant proteins ampicillin Sigma–Aldrich Cat#A9518; N/A Lipofectamine 3000 Thermo Fisher Cat#L3000015; N/A ProLong Diamond Antifade Mountant with DAPI Thermo Fisher Cat#17625062; N/A HALT Protease & Phosphatase Inhibitor Cocktail (100x) Thermo Fisher Cat#861281; N/A NUPAGE lithium dodecyl sulfate (LDS sample loading buffer Thermo Fisher Cat#NP0007; N/A dithiothreitol (DTT) reducing agent Thermo Fisher Cat#NP0004; N/A methanol-activated polyvinylidene fluoride (PVDF) membrane Merck Cat#IPFL00010; N/A tris-buffered saline (TBS) Odyssey Blocking Buffer LI-COR Biosciences Cat#92750000; N/A Perifosine Sigma-Aldrich Cat#SML0612; N/A Critical commercial assays HiSpeed Plasmid Maxi Kit Qiagen Cat#12663; N/A Pierce BCA Protein Assay Kit Thermo Fisher Cat#23227; N/A Deposited data Molecular dynamics simulation trajectories This paper https://doi.org/10.5518/1313 High resolution structure of AKT-PH Milburn et al. PDB: 1UNQ Experimental models: Cell lines .. Recombinant DNA Plasmid: AKTWT-mCherry (See Table S1) This paper N/A Plasmid: AKTWT-EGFP (See Table S1) This paper N/A Plasmid: AKTE17K-EGFP (See Table S1) This paper N/A Plasmid: AKTmut1-EGFP (See Table S1) This paper N/A Plasmid: AKTmut2-EGFP (See Table S1) This paper N/A Plasmid: AKTmut3-EGFP (See Table S1) This paper N/A Plasmid: AKT-PHmut3-mCherry (See Table S1) This paper N/A pcDNA3.1 empty vector (See Table S1) Gifted by Dr. Thomas Hughes, University of Leeds Addgene plasmid # 40728; RRID: Addgene_40728 HA-GFP reporter (See Table S1) Gifted by Dr. Thomas Hughes, University of Leeds N/A (Continued on next page) e1 Structure 33, 1–15.e1–e5, January 2, 2025

Bicinchoninic Acid Protein Assay:

Article Title: Two cooperative lipid binding sites within the pleckstrin homology domain are necessary for AKT binding and stabilization to the plasma membrane.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies AKT (pan) (40D4) mouse mAb Cell Signalling Technology Cat#2920; RRID:AB_1147620 Phospho-Akt (Ser473) (193H12) Rabbit mAb Cell Signalling Technology Cat#4058; RRID:AB_331168 b-Actin (8H10D10) Mouse mAb Cell Signalling Technology Cat#3700; RRID:AB_2242334 IRDye 800CV goat anti-mouse Cell Signalling Technology Cat#926-68170; RRID:AB_10956589 IRDye 680RD goat anti-rabbit LI-COR Biosciences Cat#926-68071; RRID:AB_10956166 Bacterial and virus strains E.coli DH10B competent cells Invitrogen Cat#1296606; N/A Chemicals, peptides, and recombinant proteins ampicillin Sigma–Aldrich Cat#A9518; N/A Lipofectamine 3000 Thermo Fisher Cat#L3000015; N/A ProLong Diamond Antifade Mountant with DAPI Thermo Fisher Cat#17625062; N/A HALT Protease & Phosphatase Inhibitor Cocktail (100x) Thermo Fisher Cat#861281; N/A NUPAGE lithium dodecyl sulfate (LDS sample loading buffer Thermo Fisher Cat#NP0007; N/A dithiothreitol (DTT) reducing agent Thermo Fisher Cat#NP0004; N/A methanol-activated polyvinylidene fluoride (PVDF) membrane Merck Cat#IPFL00010; N/A tris-buffered saline (TBS) Odyssey Blocking Buffer LI-COR Biosciences Cat#92750000; N/A Perifosine Sigma-Aldrich Cat#SML0612; N/A Critical commercial assays HiSpeed Plasmid Maxi Kit Qiagen Cat#12663; N/A Pierce BCA Protein Assay Kit Thermo Fisher Cat#23227; N/A Deposited data Molecular dynamics simulation trajectories This paper https://doi.org/10.5518/1313 High resolution structure of AKT-PH Milburn et al. PDB: 1UNQ Experimental models: Cell lines .. Recombinant DNA Plasmid: AKTWT-mCherry (See Table S1) This paper N/A Plasmid: AKTWT-EGFP (See Table S1) This paper N/A Plasmid: AKTE17K-EGFP (See Table S1) This paper N/A Plasmid: AKTmut1-EGFP (See Table S1) This paper N/A Plasmid: AKTmut2-EGFP (See Table S1) This paper N/A Plasmid: AKTmut3-EGFP (See Table S1) This paper N/A Plasmid: AKT-PHmut3-mCherry (See Table S1) This paper N/A pcDNA3.1 empty vector (See Table S1) Gifted by Dr. Thomas Hughes, University of Leeds Addgene plasmid # 40728; RRID: Addgene_40728 HA-GFP reporter (See Table S1) Gifted by Dr. Thomas Hughes, University of Leeds N/A (Continued on next page) e1 Structure 33, 1–15.e1–e5, January 2, 2025

Agarose Gel Electrophoresis:

Article Title: Akkermansia muciniphila modulates intestinal mucus composition to counteract high-fat diet-induced obesity in mice
Article Snippet: .. The reduced samples were spin filtered through a 100k MWCO amicon 0.5 filter (merck) for 15 min at 14000 g. Lithium dodecyl sulfate (LDS) loading buffer (10 μl; Thermo Fisher) was added to the samples and loaded onto a 1% vertical agarose gel cast in Tris-Glycine-SDS (TGS) buffer (Biorad). .. The reduced samples were spin filtered through a 100k MWCO amicon 0.5 filter (merck) for 15 min at 14000 g. Lithium dodecyl sulfate (LDS) loading buffer (10 μl; Thermo Fisher) was added to the samples and loaded onto a 1% vertical agarose gel cast in Tris-Glycine-SDS (TGS) buffer (Biorad).

CRISPR:

Article Title: Nonviral Gold Nanoparticle-Mediated Delivery of CRISPR-Cas9 Ribonucleoprotein and Long DNA Transgenes Into Primary Blood Cells
Article Snippet: For diameter calculations, TEM images were analyzed in ImageJ (Version: 1.54f) by measuring diameter on two axes for at least 20 AuNP. .. A 2.5-μg CRISPR-AuNP solution based on gold core mass was combined with 4X lithium dodecyl sulfate (LDS) loading buffer (Invitrogen, Waltham, MA, the United States) according to the manufacturer’s instructions and incubated for 15 min at 95°C. .. Each sample was loaded into a NuPAGE 4%–12% Bis-Tris Mini Protein Gel (Invitrogen) and run at 200 mV for 30 min. Gels were stained using SimplyBlue SafeStain (Invitrogen) imaged using iBright FL1500 Imaging System (Invitrogen) and analyzed for density using the iBright built-in gel analysis platform.

Review